Journal:

Article Title: Large-scale identification of genes implicated in kidney glomerulus development and function

doi: 10.1038/sj.emboj.7601014

Figure Lengend Snippet: Generation of GlomBase and GlomChip and identification of glomerulus-expressed and -specific genes. (A) The four glomerular cDNA libraries, their theoretical distribution of cDNA clones relative to original transcript abundance, the number of sequenced clones from each library and the total number of sequences, annotated genes and ESTs. (B) Relative abundance of different housekeeping genes in the adult standard (blue bars), adult normalized (red bars) and adult supernormalized (green bars) libraries. Eef1a1, elongation factor 1 α1; B2 m, β2 microglobulin; Gapd, Glyceraldehyde 3-phosphate dehydrogenase; Ftl1, Ferritin light chain 1; Oaz1, Ornithine decarboxylase antizyme; Rps8, 40S ribosomal protein S8. (C) Flow chart of GlomChip hybridizations. GlomChips were hybridized against labeled targets from different tissues: isolated glomeruli, non-glomerular kidney tissue (rest of kidney), brain capillary fragments, GFP-positive glomerular cells and GFP-negative glomerular cells. Stepwise comparisons between pairs of tissues provided lists of significantly upregulated (>2-fold) genes in each tissue category, or not significantly different (NS). GC: gene category.

Article Snippet: Based on test sequencing results, we attempted a total of 15 627 5′ sequence reads from the same number of random cDNA clones (custom sequencing by Incyte and Genome Vision) selected from the four libraries, which provided 14 171 sequences of 500–800 bp length (91% readability).

Techniques: Clone Assay, Labeling, Isolation

Journal:

Article Title: Large-scale identification of genes implicated in kidney glomerulus development and function

doi: 10.1038/sj.emboj.7601014

Figure Lengend Snippet: List of category 6 and 7 genes in C

Article Snippet: Based on test sequencing results, we attempted a total of 15 627 5′ sequence reads from the same number of random cDNA clones (custom sequencing by Incyte and Genome Vision) selected from the four libraries, which provided 14 171 sequences of 500–800 bp length (91% readability).

Techniques: Clone Assay, Wilms Tumor Assay, Protease Inhibitor, Ubiquitin Proteomics, Binding Assay, Sequencing, Marker, Comparison

Journal: bioRxiv

Article Title: N153-linked glycans on envelope protein protect orthoflaviviruses from antibody-mediated clearance

doi: 10.1101/2025.02.20.639387

Figure Lengend Snippet: Neutralizing activity of (A) DENV2 (n=6) and (B) DENV1 (n=5) human convalescent plasma against corresponding WT and N153Q DENV produced in C6/36, Vero or Huh-7. (C) IC 50 values of 2D22 mAb against WT DENV2 and N153Q DENV2 mutant. (D-H) IC 50 values of C10 mAb against (D) WT DENV2 and N153Q DENV2 mutant; (E) WT DENV1 and N153Q DENV1 mutant; (F) WT DENV3 and N153Q DENV3 mutant; (G) WT DENV4 and N153Q DENV4 mutant; (H) WT ZIKV and N154Q ZIKV mutant. For C to H, each data point represents mean values of an independent experiment. Dotted line indicates the detection limit of the assay. Statistical analysis was performed using Mann–Whitney U test (A) and t-test (B&C). *, p< 0.05; **, p < 0.01; ns, not significant.

Article Snippet: DENV3 C0360/94 (GenBank accession number: KJ737429.1 , cat. # NR-48800) and DENV4 703-4 (cat. # NR-48801) were both isolated in Thailand in 1994 and were sourced from the Biodefense and Emerging Infections Research Resources (BEI Resources) repository.

Techniques: Activity Assay, Clinical Proteomics, Produced, Mutagenesis, MANN-WHITNEY

Journal: Reproduction

Article Title: Myostatin is expressed in bovine ovarian follicles and modulates granulosal and thecal steroidogenesis

doi: 10.1530/rep-18-0114

Figure Lengend Snippet: Figure 2 Immunohistochemical staining of bovine ovary sections showing myostatin immunoreactivity (brown) in oocyte and granulosa cells of primordial (pF) and primary (PrF) follicles (A), secondary (SF) follicles (B,C) and in thecal (T) and granulosal (G) layers of antral follicles (AF) (D and E). Myostatin immunoreactivity was also evident in vascular smooth muscle cells (bv) (E). No staining was observed in control sections treated with normal rabbit serum instead of primary antibody (F).

Article Snippet: Effects of myostatin on granulosal and thecal steroid secretion and viable cell number Recombinant human myostatin (R&D Systems; 94% amino acid sequence homology with bovine myostatin) was added to wells to give final concentrations of 0.08, 0.4, 2, 10, 50 and 100 ng/mL in the presence and absence of gonadotropin (FSH or LH).

Techniques: Immunohistochemical staining, Staining, Control

Journal: Reproduction

Article Title: Myostatin is expressed in bovine ovarian follicles and modulates granulosal and thecal steroidogenesis

doi: 10.1530/rep-18-0114

Figure Lengend Snippet: Figure 9 Schematic diagram illustrating potential involvement of systemic and/or locally produced myostatin in the modulation of thecal and granulosal steroidogenesis.

Article Snippet: Effects of myostatin on granulosal and thecal steroid secretion and viable cell number Recombinant human myostatin (R&D Systems; 94% amino acid sequence homology with bovine myostatin) was added to wells to give final concentrations of 0.08, 0.4, 2, 10, 50 and 100 ng/mL in the presence and absence of gonadotropin (FSH or LH).

Techniques: Produced